simview

Reconstruct SiMView Images Using BigSticher

by Eric Wait
Jul 6, 2021
data-and-analysis, microscopy
imagej, scripts, simview

Overview # There are four steps to this process. First is to create a dataset file that allows BigSticher to interpret the data. Second is to run a macro that will flip the opposing views, so they are roughly aligned. Third is to find the best parameters that register the views together. Forth is to register and export the reconstructed data. Inital Setup # First we must install ImageJ and the BigStitcher plugin. ...