Understanding research compliance at Janelia when you’re not at Janelia is not always easy. But this blog post will help walk you through what to expect prior to arrival at the AIC and how we will help you prepare.
Needless to say that, just like in any type of microscopy, low signal samples in general do not work well for SIM. Besides that, there are other factors unrelated to the general signal level that will determine if a sample, either fixed or living, is suitable for SIM. The following is by no means a complete list of those factors.
The lattice light-sheet microscope (LLSM) is a powerful optical tool for observing rapidly-occurring processes in live samples. This technique offers multiple advantages over more common optical microscopy methods, like spinning-disk confocal, with improved optical sectioning, reduced photobleaching, and minimized phototoxicity. This blog post will cover the most common model systems and the steps we take to mount and adapt the sample for lattice light-sheet microscopy.