Probes for Single Molecule Localization Microscopy

The last 10 years has seen an explosion of techniques aimed at creating biological fluorescence images with unsurpassed clarity via super-resolution microscopy. Among these various techniques that circumvent the diffraction barrier, there is a group of methods that can be termed single-molecule localization microscopy (SMLM). This blog introduces the conceptual basis, and gives pointers for selecting these fluorescent tags for use in SMLM.